Transformation ofLactobacillus helveticussubsp.jugurtiwith Plasmid pLHR by Electroporation
نویسندگان
چکیده
منابع مشابه
Broad-Host-Range Plasmid by Electroporation
Methylotrophic bacteria able to grow on one-carbon compounds, such as methanol and monomethylamine, as sole carbon sources have received considerable attention because of their potential commercial applications, including the production of single-cell protein, amino acids, pyrroloquinoline quinone, and poly-3-hydroxybutyrate. The utility of methylotrophs should be increased by the use of recomb...
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A method for introducing heterologous DNA into Saccharomyces cerevisiae rapidly and efficiently by electroporation was developed. Transformant colonies appeared somewhat sooner than by the LiCl or spheroplast transformation method, and the time spent in manipulation was much less than for these two methods. The pores in the cell membrane formed by the high voltage of electroporation were reseal...
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In this work, we provide detailed instructions for transformation of Clostridium thermocellum by electroporation. In addition, we describe two schemes for genetic modification: allelic replacement-where the gene of interest is replaced by an antibiotic marker and markerless gene deletion-where the gene of interest is removed and the selective markers are recycled. The markerless gene deletion t...
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Transformation systems have been reported for a number of filamentous fungi (1) based on the procedure perfected for Neurospora crassa by Case et al. employing sphaeroplasts (2). While efficient transformation is achieved with sphaeroplasts, their preparation demands a careful monitoring of the individual steps, along with optimization of conditions for each batch of cell wall-degrading enzymes...
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Loree Heller and M. Lee Lucas Center for Molecular Delivery, University of South Florida, Tampa, FL 33612, Department of Medical Microbiology, University of South Florida, Tampa, FL 33612 __________________________________________________________________________________ * Correspondence : Loree Heller, University of South Florida, Center for Molecular Delivery, MDC16, 12901 Bruce B. Downs Blvd....
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1990
ISSN: 0002-1369
DOI: 10.1080/00021369.1990.10870177